Testicular cultivation of Treponema pallidum (Nichols strains) facilitated by sustained-release steroid administration.
نویسندگان
چکیده
Treponema pallidum cultivation is facilitated by substitution of methylprednisolone acetate suspension for hydrocortisone administration during rabbit testicular infection. Methylprednisolone suspension reduces testicular mononuclear cell infiltration and should benefit future studies of virulent T. pallidum.
منابع مشابه
Comparison of the morphology, antigenicity, and pathology of Nichols and Birmingham isolates of Treponema pallidum.
A strain of Treponema pallidum isolated from a homosexually acquired penile chancre in Birmingham in 1979 was serially passaged in rabbits and compared with the Nichols strain. The two strains appeared identical morphologically, and no gross differences were detected on antigenic analysis by two-dimensional immunoelectrophoresis. The Birmingham strain was, however, considerably less virulent fo...
متن کاملTreponema pallidum (Nichols strain) in tissue cultures: cellular attachment, entry, and survival.
The interaction of Treponema pallidum (Nichols strain) with cultured cells was investigated under aerobic conditions. Cell monolayers derived from rabbit testicular tissue extended the survival of treponemes as indicated by active motility. Large numbers of organisms rapidly attached to cultured cells. Within 3 h, one to twelve actively motile treponemes were attached to 25 to 50% of the cells....
متن کاملGenetic relationship between Treponema pallidum and Treponema pertenue, two noncultivable human pathogens.
Genetic relationships among two strains of Treponema pallidum (Nichols and KKJ) and a strain of T. pertenue were determined by measuring the degree of deoxyribonucleic acid sequence homology. The results in indicated that these three virulent, noncultivable treponemes were genetically indistinguishable. Like T. pallidum (Nichols), T. pertenue (Gauthier) had no detectable deoxyribonucleic acid s...
متن کاملAntigenic structure of Treponema pallidum, Nichols strain. II. Extraction of a polysaccharide antigen with "strain-specific" serological activity.
An ultracentrifugally homogeneous heat-stable polysaccharide preparation free from serologically reactive rabbit testicular tissue antigen, including cardiolipin, was extracted from the Nichols strain of Treponema pallidum, and found to react by complement-fixation with homologous rabbit sera but not with human syphilitic sera. In addition, the reactive "strain-specific" component was found to ...
متن کاملResequencing of Treponema pallidum ssp. pallidum Strains Nichols and SS14: Correction of Sequencing Errors Resulted in Increased Separation of Syphilis Treponeme Subclusters
BACKGROUND Treponema pallidum ssp. pallidum (TPA), the causative agent of syphilis, is a highly clonal bacterium showing minimal genetic variability in the genome sequence of individual strains. Nevertheless, genetically characterized syphilis strains can be clearly divided into two groups, Nichols-like strains and SS14-like strains. TPA Nichols and SS14 strains were completely sequenced in 199...
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ورودعنوان ژورنال:
- Journal of clinical microbiology
دوره 10 6 شماره
صفحات -
تاریخ انتشار 1979